RT Journal Article
SR Electronic
T1 Artificial olfactory system based on nanomaterials-based sensors for early detection of tuberculosis via breath test
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P498
VO 42
IS Suppl 57
A1 Morad Nakhleh
A1 Raneen Jeries
A1 Alaa Gharra
A1 Anke Binder
A1 Yoav Broza
A1 Mellisa Pascoe
A1 Keertan Dheda
A1 Hossam Haick
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P498.abstract
AB Background: Commonly used tools for the diagnosis of tuberculosis (TB) have suboptimal accuracy, remain inaccessible as they are not point-of-care, and require a sputum sample, which is often unavailable in HIV-infected persons.Aim: To evaluate the feasibility and accuracy of nanomaterials-based electro-chemical sensor array, with odor detection ability, to diagnose TB infection, via exhaled breath analysis.Methods: A case control study (n= 198) was conducted using 64 patients with culture-proven TB and 134 controls (67 were symptomatic patients who presented to a TB clinic but were culture negative on follow-up and not started on anti-TB treatment, and 67 were healthy volunteers). Exhaled breath was sampled and analyzed using an array of chemically modified, nanomaterial-based and odor detecting sensors. While using 138 randomly selected samples as a derivation set, the remaining 60 samples as the blinded validation set. The impact of HIV status, smoking, and treatment effect was also evaluated.Results: Sensitivity, specificity, Positive and Negative Predictive Values (PPV and NPV), when using a ROC-derived most accurate cutoff point, in the derivation set were, 85%,89%,78% and 92%, respectively, moreover, in validation, blind set experiment, the relevant values were 90%,92%,86% and 95% respectively. These results were un-affected by smoking habits, HIV status, and/or prior TB treatment of several days.Conclusion: These preliminary proof-of-concept data indicate that a nanomaterial-based sensors may be a non-invasive, simple and useful for the accurate diagnosis of TB, used as mobile point-of-care screening tool.