PT  - JOURNAL ARTICLE
AU  - Erkan Cakir
AU  - Emel Torun
AU  - Esin Cetin Aktas
AU  - Gulnur Deniz
TI  - Intracellular cytokine and cathelicidin secretion from monocytes and neutrophils in childhood lung tuberculosis and its relationship with vitamin D levels
DP  - 2013 Sep 01
TA  - European Respiratory Journal
PG  - P4431
VI  - 42
IP  - Suppl 57
4099  - http://erj.ersjournals.com/content/42/Suppl_57/P4431.short
4100  - http://erj.ersjournals.com/content/42/Suppl_57/P4431.full
SO  - Eur Respir J2013 Sep 01; 42
AB  - Introduction: Vitamin D3 contributes to host immune responses against Mycobacterium tuberculosis through antimicrobial peptide human CAP 18+ (cathelicidin). We aim to identify the intracelluler cytocine and cathelicidine secretion from monocytes and neutrophils of active TB in children. Material- methods: Fifteen TB patients and 15 healthy children were enrolled to the study. Patients’ biochemical parametres and 25 hydroxyvitamin D were measured. The expression of human cathelicidin, TNF α, IF-γ and IL-8 from monocytes and neutrophils were analyzed by flow cytometry method in the serum. Results: The expression of IL-8 and cathelicidin from CD14 + monocytes were significantly higher in TB patients (p=0.0001).There was no difference in IL-8 and cathelicidin expression in CD 15+ neutrophils in both groups in the appropriate concentrations of vitamin D. The expression of TNF α and IF-γ from CD14 + monocytes and CD 15+ neutrophils, in both study and control groups revealed no statistical difference.