RT Journal Article
SR Electronic
T1 Increased laminin deposition and expression of TGF-β in bronchial biopsies of children with chronic obstructive respiratory diseases
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1255
VO 42
IS Suppl 57
A1 Lenka Honkova
A1 Katarina Berankova
A1 Jiri Uhlik
A1 Petr Pohunek
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P1255.abstract
AB BACKGROUND: Airway remodeling is well studied in bronchial asthma (AB) but its specificity and role in differential diagnosis of chronic obstructive respiratory diseases (CORD) are discussed.AIM: The aim of the study was to compare histological findings in bronchial mucosa in children with AB, cystic fibrosis (CF), primary ciliary dyskinesia (PCD) and healthy subjects and to find out if they are specific for AB.METHODS: Thirty-one subjects aged 4 to 19 years (10.25 ± 3.78 yrs) examined for various clinical reasons were included in the study. There were 6 children (8.71 ± 2.91 yrs) in the control group, 16 children with AB (9.85 ± 3.34 yrs), 5 with CF (11.1 ± 3.50 yrs) and 5 with PCD (12.43 ± 4.88 yrs). Endobronchial biopsy was taken from each patient. The thickness of laminin positive layer of the basement membrane (LAM) and the number of transforming growth factor β positive epithelial cells (TGF) were studied using light microscopy and immunohistochemical staining. The correlation between LAM thickness and the number of TGF was evaluated by Spearman’s test.RESULTS: Significant increase in LAM thickness (2.5 ± 0.29 μm vs. 1.78 ± 0.17 μm, p < 0.01) was found in children with CORD compared to controls. No significant differences were found between AB, CF and PCD groups. The correlation between LAM thickness and the number of TGF was not statistically significant (p > 0.05). This may be due to a small number of subjects.CONCLUSION: Remodeling of bronchial mucosa characterized by significant increase in LAM thickness can be found in children with several CORD and is not specific for asthma.Supported by GAUK 360213 and IGA MZ NT/11444.