TY - JOUR T1 - LSC 2013 abstract - A comparative analysis of bacterial phylotypes of the lung microbiota of healthy, IPF and transplanted lungs JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - OP01 AU - John Erb-Downward AU - Robert Dickson AU - MeiLan Han AU - Christine Freeman AU - Zachary Britt AU - Natalie Walker AU - Vibha Lama AU - Jeffery Curtis AU - Fernando Martinez AU - Gary Huffnagle Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/OP01.abstract N2 - Rationale: Culture-independent microbiology has identified a microbiota in the lungs of both healthy and diseased individuals, which may provide novel insights into the progression of lung disease.Methods: Bronchoalveolar lavage (BAL) samples (26 healthy, 44 lung transplant and 78 IPF) were obtained for microbiome analysis by 454-pyrosequencing the V5-V3 region of the bacterial 16S rRNA gene. The software package mothur was used to process, cluster and assign sequences to phylotyped operational taxonomic units (OTUs). Community analysis was carried out in R using the R-package vegan.Results: Bacterial communities were visualized by ordination and clusters identified, based on sample type. The lung microbiota of IPF patients and that of transplanted lungs was significantly different from normal lungs (p = 0.001). A non-linear multidimensional scaling of the data demonstrated that the IPF samples could also be broken up into two groups: one that overlapped with the healthy controls and one distinct group. Analysis of the dominant OTUs in each population demonstrated that the lung community of healthy controls, and the overlapping IPF subgroup, was dominated by the genera Prevotella and Veillonella.The other IPF subgroup was dominated by the genera Pseudomonas, Clostridium XI group, Prevotella and Flavobacterium. The community of the transplant group was dominated by the genera Pseudomonas, Prevotella and Escherichia.Conclusions: The lung microbiota of IPF patients and lung transplants is significantly different than the lung microbiota of healthy controls. ER -