RT Journal Article
SR Electronic
T1 LSC 2013 abstract - Identification of biomarkers for early diagnosis in BPD
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP PP112
VO 42
IS Suppl 57
A1 Tina Gimm
A1 Anita Hoeland
A1 Maren Schwarz
A1 Hamid Hossain
A1 Harald Ehrhardt
A1 Ludwig Gortner
A1 Markus Scholz
A1 Tobias Reicherzer
A1 Stefanie Hauck
A1 Anne Hilgendorff
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/PP112.abstract
AB Rationale: Bronchopulmonary dysplasia (BPD), one of the most common complications in the preterm infant, is characterized by impaired alveolar and vessel development. Mechanical ventilation, oxygen toxicity and infections increase the risk for BPD with impaired pulmonary and neurologic outcome persisting into adulthood. Current definitions rely on late pulmonary function and reliable biomarkers allowing for an early diagnosis are still missing.Objective and Methods: We therefore subjected cord blood (CB) from preterms < 32 weeks gestational age with and without BPD to microarray (Code Link, cohort 1) and cytokine analysis (Luminex®, cohort 2) for identification of signaling pathways and potential candidates for biomarker analysis. The results were correlated with data from fibroblasts isolated from tracheal aspirates (TA). First results from proteome analysis in urine and TA (LC-MS/MS) were compared to the results obtained by microarray and cytokine analysis.Results: Transcriptome analysis exhibited 102 up- and 134 down-regulated genes in preterms with BPD compared to no-BPD controls. Genes were involved in regulation of gene expression (up), or cell differentiation, embryonic development and ion homeostasis (down). Luminex analyses revealed significant differences in cytokines (sIL-1ra, IL-8, TNF-a, MCP-1) and growth factor expression (FGF2, EGF), correlating with functional data in fibroblasts from TA. First proteome data in urine and TA confirmed potential biomarker candidates.Conclusion: Gene expression and protein profiling from CB, urine and TA of preterms allows differentiation for later BPD diagnosis with increased monocyte signaling and fibroblast activation playing a central role.