TY - JOUR T1 - Distribution of vitamin C transporters in the lower airways of humans JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - 5006 AU - G.D. Rankin AU - N. Larsson AU - E. Roos-Engstrand AU - J. Pourazar AU - A. Blomberg AU - I. Mudway AU - A.F. Behndig Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/5006.abstract N2 - Vitamin C (ascorbate, AscH2 + dehydroascorbate, DHA) is an important low-molecular weight antioxidant at the air-lung interface. Despite its critical role, little is known about its transport and the regulation of intra-cellular concentrations in the lung. Whilst several vitamin C transporters are identified, such as sodium-ascorbate co-transporters (SVCT1/2) and glucose transporters (GLUTs), the latter transporting the AscH2 oxidation product DHA, knowledge of their presence and distribution in the human lung is limited (GLUTs) or unknown (SVCTs).We hypothesised that these transporters are present within the bronchial airways and play a vital role in the regulation of epithelial and respiratory tract lining fluid vitamin C concentrations.We investigated the distribution of these transporters in endobronchial biopsies obtained by bronchoscopy from 16 mild asthmatics and 16 age-matched healthy controls.Positive staining was found in blood vessels for SVCT1 and 2; with SVCT 2 and GLUT2 also present in the apical epithelium. Staining with GLUT1 yielded a distinct cell membrane staining. There were no apparent differences between asthmatics and controls. SVCT2 staining was further shown to be predominately localised to goblet cells. SVCT2+goblet cells were negatively correlated with total vitamin C concentrations determined in the bronchial wash of these subjects (ρ=-0.661, p<0.05).This is the first study to demonstrate that SVCT2 is present in the human lung epithelium, localised mainly within goblet cells. The negative correlation between SVCT2+goblet cells and vitamin C suggests that these cells may play a hitherto unknown function in vitamin C re-uptake and recycling at the air-lung interface. ER -