RT Journal Article
SR Electronic
T1 Leukocyte migration induced by intestinal ischemia and reperfusion in obese mice
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P638
VO 42
IS Suppl 57
A1 Evelyn Fantozzi
A1 Maria-Beatriz Amorim
A1 Fernanda Silva
A1 Daniel Romero
A1 Ana Breithaupt-Faloppa
A1 Ricardo Oliveira-Filho
A1 Boris Vargaftig
A1 Domenico Spina
A1 Yanira Riffo-Vasquez
A1 Wothan Tavares-de-Lima
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P638.abstract
AB There does not appear to be a general consensus whether obesity confers protection or exacerbates acute lung injury. In this study we have investigated leukocyte migration to the lung induced by intestinal ischemia/reperfusion (I/R) injury in obese mice in vivo and in vitro. Method Female mice were fed with high fat (hfd) or standard diet (sd) for 90 days. We measured levels of cholesterol and triglycerides, glycemic curve, abdominal fat and weight gain. Ischemia was performed by occlusion of the superior mesenteric artery for 45 min followed by a 2h reperfusion. The percentage of PMN present in the lung tissue after digestion with collagenase and number of leukocytes in the bone marrow was measured. Bone marrow cell chemotaxis in response to MDC was measured using a modified Boyden chamber. Results Mice fed with a high fat diet showed a significant weight gain (Δ) over 90 days in comparison to lean mice (sd=0.89±0.4; hfd=3.5±0.8 g, n=10; p<0.05), higher abdominal fat gain (sd=0.33±0.02; hfd=1.26±0.13 g, n=10), lower glucose uptake (sd=224.1±14.1; hfd=375.5±30.8 mg/dl, n=10), higher cholesterol (sd=65.5±1.3; hfd=118.1±3.7 mg/dl, n=7; p<0.05), and triglyceride levels (sd=42.0±4.0; hfd =60.2±13.0 mg/dl, n=9). Lower percentage of PMN was found in the lung of obese mice submitted to I/R (sd I/R: 36.06±1.55, hfd I/R: 28.25±1.68, %, n=7). Obese mice had lower number of leukocytes in the bone marrow after I/R in comparison to lean mice (sd I/R=14.88±2.5; hfd I/R=5.0±0.82, x106cells/ml, n=13; p<0.05) and less cells migrated in response to MDC in vitro (sd I/R=31.5±4.3; hfd I/R=20.3±4.5 x106cells/ml, p<0.5, n=8). Conclusion Our results suggest that obesity impairs leukocyte migration to the lung.