RT Journal Article
SR Electronic
T1 The protein DJ-1 in malignant pleural effusions due to lung cancer: Levels and correlations with oxidative stress biomarkers
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P2914
VO 42
IS Suppl 57
A1 George Vavougios
A1 Theodora Kerenidi
A1 Irene Tsilioni
A1 Konstantinos Gourgoulianis
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P2914.abstract
AB BACKGROUND: DJ-1 is a multifunctional protein in lung cancer and a regulator of the oxidative stress response in pneumonocytes.AIM: The aim of the present study was to determine DJ-1s levels in malignant pleural effusions (MPEs) and detect possible correlations with oxidative stress biomarkers.METHODS: Forty lung cancer patients, mean age 65±10 years with cytologically proven MPEs were enrolled in this study. Pleural fluid (PF) and peripheral blood samples were collected, followed by a routine analysis of biochemical parameters. DJ-1, Cu/Zn-superoxide dismutase-1 (SOD1) and 8-Isoprostane levels in PF samples were measured by enzyme-linked immunosorbent assay (ELISA) kits.RESULTS: The median value of DJ-1's levels was 826 ng/mL (Interquartile Range: 482 - 1010 ng/mL). DJ-1 levels correlated with SOD1 and 8-Isoprostane levels (Spearman's rho =-0.476, P=0.002 and Spearman's rho =-0.264, P=0.033, respectively). Furthermore, DJ-1 levels correlated with PF lactate dehydrogenase (LDH, Spearman's rho =-0.497, P=0.001), LDH PF to blood ratio (Spearman's rho =-0.392, P=0.012) and total cell count (Spearman's rho =-0.325, P=0.041). Finally, patients over 65 years displayed higher DJ-1 levels than younger patients (875 ng/mL vs 607 ng/mL respectively, P= 0.037).CONCLUSIONS: DJ-1's correlations with oxidative stress biomarkers may imply the presence of a pathophysiological mechanism of antioxidant regulation intrinsic to MPEs due to lung cancer.