PT  - JOURNAL ARTICLE
AU  - Michael Crooks
AU  - Alyn Morice
AU  - Khalid Naseem
AU  - Simon Hart
TI  - Platelet-endothelial adhesion in IPF
DP  - 2013 Sep 01
TA  - European Respiratory Journal
PG  - P473
VI  - 42
IP  - Suppl 57
4099  - http://erj.ersjournals.com/content/42/Suppl_57/P473.short
4100  - http://erj.ersjournals.com/content/42/Suppl_57/P473.full
SO  - Eur Respir J2013 Sep 01; 42
AB  - Background Epidemiological studies have demonstrated an association between IPF and vascular disease. The recognised role of platelets in vascular disease and their profibrotic potential led us to investigate platelet reactivity in IPF. We previously reported increased platelet reactivity due to a plasma factor in IPF. In this study we investigate platelet-endothelial adhesion.MethodBlood was collected from 10 IPF patients and 10 controls to prepare platelet poor plasma. Washed control platelets were suspended in autologous plasma, allogeneic control plasma or IPF patient plasma and labelled with antiCD42b, stimulated with ADP and incubated with HUVEC. The percentage of endothelial cells with one or more adherent platelet(s) were identified by flow cytometry.ResultsPlatelet-endothelial adhesion was significantly greater following incubation in IPF plasma compared with autologous and allogeneic control plasma at basal levels (0.86±0.03%, 0.09±0.03% and 0.15±0.03% respectively. P <0.05) and following stimulation with 0.1μM ADP (0.88±0.3%, 0.16±0.04% and 0.12±0.04%. P <0.05) and 10μM ADP (0.71±0.21%, 0.26±0.08% and 0.34±0.1%. P <0.05)(Figure 1).ConclusionPlatelets incubated in IPF patient plasma have an increased propensity to adhere to endothelial cells providing further evidence of an abnormal platelet response in IPF patients and demonstrating a potential mechanism by which platelets may contribute to pulmonary fibrosis.Figure 1. Platelet-endothelial adhesion. * P <0.05.