TY - JOUR T1 - Genes affecting mRNA stability and metalloproteinase inhibitor RECK are down-regulated in patients with COPD JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - 3512 AU - Zdenka Navratilova AU - Tereza Krsjakova AU - Eva Novosadova AU - Jaromir Zatloukal AU - Vitezslav Kolek AU - Martin Petrek Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/3512.abstract N2 - The local lung inflammatory response in chronic obstructive pulmonary disease (COPD) has proteolytic nature with elevated activity of matrix metalloproteinases (MMPs). As with other genes, the expression of MMPs and their inhibitors is affected by mRNA stability. Our aim was, therefore, to investigate the expression of two inhibitors of MMPs (RECK and PTEN) and of RNA-binding proteins affecting mRNA stability (AUF1, HuR and PCBP2) in bronchoalveolar cells from COPD patients. Real time-PCR method with PSMB2 as reference gene was used to quantify the relative expression of the tested genes in 11 non-smoking controls, 11 smoking controls, 8 non-smoking COPD patients and 22 smoking patients. The relative expression of RECK was decreased in COPD patients compared to healthy controls (p=0.004). Sub-analysis among the four subject groups stratified based on their smoking status showed that the RECK expression was decreasing gradually in the direction from non-smoking controls to smoking COPD patients (p=0.01). Of RNA-binding proteins, the numbers of AUF1 (p=0.01) and HuR (p=0.03) mRNA transcripts were lower in COPD patients than those in healthy control subjects. There was a positive correlation between RECK expression and AUF1 (p =0.001), PCBP2 (p =0.001) and HuR (p=0.02), respectively. In conclusion, altered expression of the MMPs inhibitor and deregulated mRNA stability observed in this study may be involved to COPD pathogenesis. Further studies are required to clarify the relationship between RECK and RNA-binding proteins in COPD.Acknowledgment: Grant support IGA PU LF 2013_009 and CZ.1.05./2.1.00/01.0030. ER -