TY - JOUR T1 - Detection of nontuberculous mycobacteria in Siberian region JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - P4421 AU - Olga Alkhovik AU - Maya Dymova AU - Tatyana Petrenko Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/P4421.abstract N2 - Introduction: Nontuberculous Mycobacteria (NTM) are ubiquitous in the environment, particularly in water and soil. Among them, there are dangerous and colonizing species. Mycobacteria can cause infection in humans and animals. NTM infection may be difficult to differentiate from colonization, and when NTM infection is diagnosed, it is not a reportable disease. NTM infection case-reporting data in the Siberian Federal Region of Russia in the past five years remain stable at low level: in 2009-0.2%, 2011-0.1%, 2010-0.3% of the total number of studies on identifications.Objective: To estimate NTM prevalence in Siberian Region.Methods: microbiological test, sequencing, GenoType® Mycobacterium CM/AS (“Hain Lifescience”).Results: We detected 36 different strains of NTM, isolated from patients with pulmonary tuberculosis. Species composition was as following: M.peregrinum - 8 isolates, M.fortuitum-8, M.gordonae -4, M.intracellulare-3, M.abscessus-4, M.kansasii-3, M.thermoresistibile-1, M. murale-1, M.arupense-1, M.smegmatis-2, M.chelonae-1. Results of all the above tests were the same.Conclusions: NTM disease is clearly a major problem in certain groups, including patients with underlying lung disease and also in individuals with impaired immunity. Accordingly, a thorough diagnosis of NTM disease is necessary. The rates of NTM infection and disease are increasing, thereby the problem will likely continue to grow and become a far more important issue than current rates suggest. The capacity of laboratory diagnosis for mycobacteriosis in Siberian Region is not sufficient; therefore it is necessary to create a common collection of strains with known biochemical attributes, and culture and genotyping information. ER -