RT Journal Article
SR Electronic
T1 The crucial role of IL-10 in the anti-inflammatory effects of aerobic exercise in a model LPS-induced ARDS
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P4402
VO 42
IS Suppl 57
A1 Rodolfo P. Vieira
A1 Manoel C. Oliveira-Junior
A1 Ricardo W Teixeira
A1 Flavia R. Greiffo
A1 Vanessa R. Silva
A1 Paulo R. Pereira
A1 Adilson S.A. Sousa
A1 Regiane Albertini
A1 Luis V.F. Oliveira
A1 Ana Paula Ligeiro-Oliveira
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P4402.abstract
AB Recent studies show that low intensity aerobic exercise reduces lung inflammation. These studies demonstrated that anti-inflammatory effects of exercise are mediated by IL-10. Therefore, aiming to investigate the role of IL-10 mediating the exercise effects in the pulmonary inflammation, this study used IL-10 -/- mice subjected to 5 weeks of low intensity aerobic exercise treadmill training in a model of acute inflammation induced by LPS (10ug/mouse intra-tracheally). C57Bl/6 IL-10 +/+ and IL-10 -/- male mice (n = 5) were distributed in Control (IL-10 +/+), Exercise (IL-10 +/+), LPS a (IL-10 +/+ + LPS), LPS b (IL-10 -/- + LPS), LPS + Exercise a (IL-10 +/+) and LPS + Exercise b (IL-10 +/+ + LPS). The results showed that LPS in IL-10 +/+ mice presented increased number of neutrophils in BALF and in lung parenchyma, and also increased levels of IL-1beta, IL-6 and TNF-alpha in BALF and serum compared with Control group (p0.001). In IL-10 -/- mice, LPS showed still higher number of neutrophils in BALF and in lung parenchyma, and also increased levels of IL-1beta, IL-6 and TNF-alpha in BALF and serum when compared with IL-10 +/+ mice (p0.01). On the other hand, exercise in LPS stimulated IL-10 +/+ mice reduced the number of neutrophils in BALF and in lung parenchyma, as well as the levels of IL-1beta, IL-6 and TNF-alpha, while increased the levels of IL-10 (p0.01). On contrary, IL-10 -/- mice stimulated with LPS and subjected to exercise, did not present reduction of neutrophils in BALF and parenchyma and IL-1beta, IL-6 and TNF-alpha levels. Therefore, we conclude that IL-10 is essential for the anti-inflammatory effects of aerobic exercise on acute lung inflammation.