RT Journal Article
SR Electronic
T1 Immune, metabolic and cytokine status in allergic rhinitis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P890
VO 42
IS Suppl 57
A1 Oksana Kolenchukova
A1 Svetlana Smirnova
A1 Andrey Savchenko
A1 Sergey Tereshchenko
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P890.abstract
AB Aim: To determine immune, metabolic and cytokine status in patients with allergic rhinitis (AR).Material and Methods: We studied 37 patients with AR and 71 healthy in ages from 25 to 45 years. Subpopulation composition in blood lymphocytes was evaluated by ductal cytometry using monoclonal antibodies. We determined Ig (A, M, G, E), concentration of circulating immune complexes (CIC). Cytokin status was determined by ELISA. The activity of NAD(P)-dependent dehydrogenases in lymphocytes by bioluminescent method.Results: We found in AR increase of leukocytes, NK and B-cells. We marked the lowering of lymphocytes, T-cells, CD4+ and CD4+/CD8+ compared to control. CIC increase compared to control. Cytokine status showed higher of IL-1β, IL-4, IL-6, IL-8 in serum and nasal washout in AR compared to control. In AR we determined higher INF-α in serum and in nasal washout against control.We marked the increase of the activity in such enzymes as G6PDH, NADP-dependent reaction of MDH, NAD-dependent reactions of MDH and LDG and NADH-dependent reaction of MDH and decrease in the level of such enzymes as G3DH, NADP-dependent reaction of GDH and NADPH-dependent reaction of GDH in AR compared to control.Conclusion: We should mention an important role of metabolic processes in immune system cells in homeostasis. Speed and intensity of immune reactions depend on the initial state of lymphocyte metabolism. The changes in the activity of intracellular enzymes show the increase of intensity of energy and plastic processes in blood lymphocytes in AR as compared to control.