RT Journal Article
SR Electronic
T1 Brain oxidative stress induced by recurrent obstructive apneas: Old versus young rats
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P316
VO 42
IS Suppl 57
A1 Mireia Dalmases
A1 Anna Planas
A1 Marta Torres
A1 Leo Marquez-Kisinousky
A1 Isaac Almendros
A1 Cristina Embid
A1 Miguel Angel Martinez
A1 Ramón Farre
A1 Josep Maria Montserrat
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/P316.abstract
AB Background:Obstructive sleep apnea (OSA) is over 50% in elderly population and cognitive impairment is a well-known consequence. It has been reported that both oxygen partial pressure (PtO2) and oxidative stress increase during recurrent apneas in brain tissue of young rats(Almendros et al. Sleep, 2011;34:1127–1133). It has also been recently demonstrated a lower mean increase and a decrease in PtO2 swings amplitude during apneas in older rats (ATS,2013), but it is not known whether this pattern translates into brain oxidative stress. Aim: To measure the brain oxidative stress in response to obstructive apneas in young and old rats.Methods: This study was carried out on 40 male Wistar rats: 20 young (12-weeks old) and 20 old (18-months old). The rats were non-invasively subjected to recurrent obstructive apneas (50 apneas/h, 15 s each) over 4 hours. Brain tissue samples were excised for measurement of the content of lipid peroxidation (LPO), using an Assay Kit, and antioxidant enzyme levels were determined by Western Blot.Results: LPO significantly increased after apneas in young (p<0.01) but not in old rats. Superoxide dismutase, glutathione peroxidise-1, and gluthatione reductase increased after apneas, especially in the old rats (p<0.05).Conclusion: The results show that, after recurrent apneas mimicking OSA, brain oxidative stress in older rats is lower than in young ones, probably due to increased levels of antioxidants.