TY - JOUR T1 - Phenotypic plasticity of alveolar macrophages in pulmonary sarcoidosis JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - 3066 AU - Svetlana Lyamina AU - Natalia Demianenko AU - Svetlana Shelukhina AU - Olga Lovacheva AU - Eugeny Shmelev AU - Igor Malyshev Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/3066.abstract N2 - Microenvironment to a great extent determinates alveolar macrophages (AM) phenotype. Influenced by factors of microenvironment AM can change their phenotype and affect the balance of M1/M2 phenotypes and Th1/Th2 components of the immune system, respectively. Objective: assessment of AM phenotypic plasticity in pulmonary sarcoidosis (PS) of various state and healthy volunteers (HV). Methods: In vitro experiments were carried out on AM cultures of patients with newly diagnosed PS (n=15, 41,72±3,89 y.o.), recurrent PS (n=15, 44,10±5,06 y.o) and HV (n=10, 51,83±3,52 y.o.). AM phenotype was assessed by flow cytometry (Beckman Coulter, FC500) by surface macrophages markers typical for M1 (CD80, CD25) and M2 (CD163, CD206) phenotype. Phenotypic plasticity of AM was measured as percentage change of markers during 36 hours of AM reprogramming in the presence of 0%, 10%, 40% standard fetal bovine serum, containing endogenous reprogramming factor – surfactant protein D. Results: Total phenotypic plasticity of AM in PS was decreased compared to HV. The more expressed changes were observed in CD80 (M1) and CD206 (M2). In newly diagnosed PS expression of CD80 was 1.5 times lower and in recurrent PS – 3.5 times lower than in HV. In newly diagnosed and recurrent PS expression of CD206 was 1.4 and 1.6 times lower than in HV, respectively. There were no significant changes in expression of CD163. Conclusions: The capability of AM to change their phenotype under the influence of microenvironment in PS of various state was decreased compared to healthy volunteers. Total phenotypic plasticity of AM in newly-diagnosed PS exceeded the results in recurrent sarcoidosis but was decreased compared to healthy volunteers. ER -