RT Journal Article
SR Electronic
T1 Ghrelin inhibits interleukin-6 production induced by cigarette smoke extract in the bronchial epithelial cells via NF-κB pathway
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4874
VO 42
IS Suppl 57
A1 Ting Yang
A1 Yongchun Shen
A1 Xiaoou Li
A1 Fuqiang Wen
A1 Liuqun Jia
A1 Chun Wan
YR 2013
UL http://erj.ersjournals.com/content/42/Suppl_57/4874.abstract
AB Objectives: The present study was designed to evaluate whether ghrelin inhibits the proinflammatory cytokine interleukin-6 (IL-6) productiion induced by cigarette smoke extract (CSE) in the human bronchial epithelial cell line (16-HBE) and which mechanism is related with this effect of ghrelin.Methods: 16-HBE cells were preincubated with vehicle or ghrelin (0.1 to 1000 ng/mL) in a concentration-dependent manner and then CSE (0 to 16%) was added. The secretion and mRNA expressions of IL-6 was detected by ELISA and RT-PCR. The phosphorylation of IKKα/β/p65 protein and the degradation of inhibitory protein-κB (I-κB) in 16-HBE were examined by western blot analysis, and then detected the generation of reactive oxygen species (ROS) in 16-HBE by labeling pecific fluorescence probes DCFH-DA.Results: Cells treated with CSE (8%) exhibited significantly higher IL-6 production and secretion compared with cells treated with vehicle alone (P<0.05). Ghrelin inhibited CSE-induced IL-6 production by 16-HBE at both mRNA and secretive levels in a concentration-dependent manner (P<0.05). Moreover, ghrelin attenuated CSE-triggered NF-κB activation in 16-HBE (P<0.05), but the intracellular ROS level after application of CSE was not affected by ghrelin (1000 ng/mL) (P>0.05).Conclusions: Together, these results suggest that ghrelin inhibits CSE-induced IL-6 production in 16-HBE cells by targeting on NF-κB pathway, but not by scavenging intracellular ROS.