TY - JOUR T1 - Effect of ambroxol and beclomethasone on LPS/TNF-α-induced neutrophil-related chemokines and nitrative stress in human bronchial epithelial cells JF - European Respiratory Journal JO - Eur Respir J VL - 42 IS - Suppl 57 SP - 1766 AU - Fabio L.M. Ricciardolo AU - Valentina Sorbello AU - Sabrina Benedetto AU - Davide Paleari Y1 - 2013/09/01 UR - http://erj.ersjournals.com/content/42/Suppl_57/1766.abstract N2 - Ambroxol (AM) protects against oxidative stress and beclomethasone dipropionate (BDP) is an anti-inflammatory drug.We evaluated the ability of AM and/or BDP to inhibit the neutrophil-related chemokines IL-8, RANTES, NAP-2 and iNOS expression in human bronchial epithelial cells (BEAS-2B). Effects on MPO and 3-nitrotyrosine (3-NT) in BEAS-2B co-cultured with human neutrophils (HN) were evaluated.BEAS-2B, alone or with HN, were stimulated with LPS (1 μg/μl) or TNF-α (10 μg/ml). IL-8, RANTES, NAP2 and iNOS expression was assessed by Western blot; IL-8, MPO and 3-NT release was measured by ELISA.In BEAS-2B LPS increased RANTES, NAP-2 and iNOS expression (p<0.01) and IL-8 release (p<0.05). TNF-α overexpressed IL-8 (p<0.01). HN co-cultured with BEAS-2B stimulated IL-8 and 3-NT (p<0.01) or slightly induced MPO and the addition of LPS or TNF-α potentiated these effects (p<0.05). AM inhibited RANTES and iNOS expression and IL-8, MPO and 3-NT release in BEAS-2B alone or with HN (p<0.05). BDP reduced NAP-2 and iNOS expression and IL-8, MPO and 3-NT release in BEAS-2B alone or with HN (p<0.05). The association of AM and BDP potentiated the inhibition on IL-8 and 3-NT (p<0.05).This study showed that AM inhibited either neutrophilic activity or nitrative stress in vitro. The synergistic effect of AM and BDP on IL-8 and 3-NT suggests new therapeutic options in the treatment of neutrophilic-related respiratory diseases like chronic bronchitis/COPD. ER -