%0 Journal Article %A Alvaro Giron-Martinez %A Terron-Exposito Raul %A Sandra Perez-Rial %A Nicolas Gonzalez-Mangado %A Peces-Barba German %T Liver growth factor (LGF) reverts experimental lung emphysema induced by tobacco smoke in mice %D 2012 %J European Respiratory Journal %P P4597 %V 40 %N Suppl 56 %X The liver growth factor (LGF) is a hepatic mitogen able to modulate the remodeling induced in several diseases, as hypertension in the arteries or cirrhosis in the liver. Considering the therapeutic effect of the LGF on the experimental COPD model induced by CdCl2 (Arch. Bronconeumol. 2010; 46 (1:20-26), we aimed to study the regenerative effects of this factor over the experimental COPD tobacco induced model.15 mice C57BL/6j were exposed to cigarette smoke or sham smoke during 6 month, twice a day and five days a week. After last exposition the treated group received 1.7 µg of LGF i.p. in two administrations (1/wk).Variables of lung morphometry (mean linear intercept –LM-), lung function (maximal volume at 30 cmH2O –Vmax-) and in vivo fluorescence molecular imaging (FMI) with the MMPsense680 probe that detects activation of matrix metalloproteases (MMPs-4-3-9-13) were analyzed in this study.The tobacco smoke group developed lung emphysema as estimated by the LM value (41.88±0.64 µ vs 32.46±0.46 µ in the control group. p<0.01) and the Vmax (1.391±0.15 ml vs 1.287±0.149 ml. p<0.05). The LGF treated group significantly improved both values reaching the control values (LM 32.04±0.35 µ. p<0.01; Vmax 1.308±0.149 ml. p<0.05). In vivo FMI of MMPs activation showed a significative increasing in the tobacco smoke group (1.83±0.1 AU from respective control. P<0.01). After LGF treatment, in vivo FMI was significantly reduced also reaching the control level (p<0.01).In conclusion, experimental lung emphysema developed by chronic exposition to cigarette smoke in C57BL/6j was reverted by LGF treatment, as estimated by MMPs activity, lung morphometry and lung function. %U https://erj.ersjournals.com/content/erj/40/Suppl_56/P4597.full.pdf