RT Journal Article
SR Electronic
T1 High incidence of the Beijing strains among multi drug resistance isolates of Mycobacterium tuberculosis from extra pulmonary tuberculosis cases in northern India
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P2718
VO 40
IS Suppl 56
A1 Anand Kumar Maurya
A1 Surya Kant
A1 Vijaya Lakshmi Nag
A1 Ram Awadh Singh Kushwaha
A1 Tapan N. Dhole
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P2718.abstract
AB Background: The emergence and spread of drug resistance tuberculosis has worsened the global resurgence of tuberculosis. Beijing strains are responsible for massive spread and outbreaks of Multidrug resistance Mycobacterium tuberculosis (MDR-TB) in worldwide as well as in India.Objectives: The aim of study presented here was to investigate incidence of Beijing genotypic among MDR-TB isolates from extra pulmonary tuberculosis cases (EPTB) in Northern India.Methods: A total of 756 specimens from patients of EPTB cases with varied presentation were studied. A total of 164 M. tuberculosis complex (MTBC) isolates recovered during the period Sept 2007-Dec 2010 were tested for drug susceptibility against SHRE by radiometric BACTEC method. MDR-TB isolates were sequenced in rpoB and katG gene for mutation analysis. All MDR-TB strains were processed by new multiplex polymerase chain reaction (PCR) for identification of Beijing strains and non Beijing strains.Results: Of these 164 MTBC, 100(60.9%) strains were fully susceptible and 64(39.1%) strains were resistance. 21 (12.8%) strains were confirmed MDR-TB by genotypic method. The proportional of Beijing strains was significantly higher among MDR-TB strains (72.7%, p < 0.05). Genotypic analysis of rpoB gene revealed significantly higher rate of Ser531Leu mutation rate among Beijing vs. non Beijing strains (50% vs. 33.4%, p < 0.05). While mutation for Ser315Thr in katG gene was common among Beijing vs. non Beijing strains (68.2% vs. 50%, p < 0.05).Conclusion: We found high incidence of Beijing strains among MDR-TB strains from EPTB cases in Northern India.