PT  - JOURNAL ARTICLE
AU  - Anand Kumar Maurya
AU  - Surya Kant
AU  - Vijaya Lakshmi Nag
AU  - Ram Awadh Singh Kushwaha
AU  - Tapan N. Dhole
TI  - High incidence of the Beijing strains among multi drug resistance isolates of Mycobacterium tuberculosis from extra pulmonary tuberculosis cases in northern India
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P2718
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P2718.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P2718.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Background: The emergence and spread of drug resistance tuberculosis has worsened the global resurgence of tuberculosis. Beijing strains are responsible for massive spread and outbreaks of Multidrug resistance Mycobacterium tuberculosis (MDR-TB) in worldwide as well as in India.Objectives: The aim of study presented here was to investigate incidence of Beijing genotypic among MDR-TB isolates from extra pulmonary tuberculosis cases (EPTB) in Northern India.Methods: A total of 756 specimens from patients of EPTB cases with varied presentation were studied. A total of 164 M. tuberculosis complex (MTBC) isolates recovered during the period Sept 2007-Dec 2010 were tested for drug susceptibility against SHRE by radiometric BACTEC method. MDR-TB isolates were sequenced in rpoB and katG gene for mutation analysis. All MDR-TB strains were processed by new multiplex polymerase chain reaction (PCR) for identification of Beijing strains and non Beijing strains.Results: Of these 164 MTBC, 100(60.9%) strains were fully susceptible and 64(39.1%) strains were resistance. 21 (12.8%) strains were confirmed MDR-TB by genotypic method. The proportional of Beijing strains was significantly higher among MDR-TB strains (72.7%, p < 0.05). Genotypic analysis of rpoB gene revealed significantly higher rate of Ser531Leu mutation rate among Beijing vs. non Beijing strains (50% vs. 33.4%, p < 0.05). While mutation for Ser315Thr in katG gene was common among Beijing vs. non Beijing strains (68.2% vs. 50%, p < 0.05).Conclusion: We found high incidence of Beijing strains among MDR-TB strains from EPTB cases in Northern India.