TY - JOUR T1 - Early signs of oxidative stress in COPD JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P3774 AU - Helen Evsyukova AU - Marina Palay AU - Anna Vjushina Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P3774.abstract N2 - Oxidative modification of proteins is the early and most reliable sign of oxidative stress which plays an important role in pathogenesis of COPD. We studied the oxidative modification of proteins in 12 patients with COPD at the exacerbation of the disease, 12 patients at the remission of COPD and 24 healthy subjects. It was shown that COPD patients had an increased amount of carbonyl derivatives originating at the initiation of oxidation amino acid rests of protein at the exacerbation (0.05±0.01U/mg) and remission stage (0.03±0.00U/mg) than in control subjects (0.01±0.00U/mg) (p<0.05).COPD patients had a decreased amount of carbonyl derivatives at the stage of elongation of the process. This may be due to the considerable protein destruction. We have shown low level of reduced thiols at the exacerbation (0.41±0.03U/mg) and remission stage (0.33±0.03U/mg) in comparison with control subjects (0.52±0.01U/mg) (p<0.001) which points out to the exhaustion of antioxidant system. The data received correlate with disruption of pulmonary function and oxygen saturation in COPD patients. ER -