RT Journal Article
SR Electronic
T1 Proinflamatory gene polymorphism and fatigue in sarcoidosis
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P778
VO 40
IS Suppl 56
A1 Tatjana Radjenovic Petkovic
A1 Tatjana Pejcic
A1 Desa Nastasijevic Borovac
A1 Milan Rancic
A1 Ivana Stankovic
A1 Lidija Ristic
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P778.abstract
AB Introduction: Fatigue is one of the most prominent symptoms in sarcoidosis patients. Cause of fatigue in sarcoidosis is still unknown. No relationship was found between fatigue and disease activity marker in sarcoidosis. Fatigue can persist many years after disease remission. Proinflammatory cytokines, TNF-α and LT-α, play important role in sarcoidosis pathogenesis. Elevated values of TNF-α and LT-α, are detected in patients with chronic fatigue syndrome of unknown etiology. Aim of our study was to investigate possible association between TNF-α and LT-α gene polymorphism and fatigue in sarcoidosis patients.Methods: One hundred and twenty eight patients with sarcoidosis were included in study. Fatigue was measured on the Fatigue Assessment Scale (FAS). Genetic tests were performed using the patients' peripheral blood. Instances of polymorphism were examined by PCR-RFLP (polymorphism detection based on the restriction fragment length) on the DNA isolated from the blood by the commercial kit (Fermentas, Lithuania). Fatigue was present in 71 patients.Results: Our results showed significantly higher frequency of rare LTα+252 G allele (29,58% vs. 19,30%, p<0,05), as well as homozygous GG (9,85% vs. 8,77%) and heterozygous GA genotype (39,44% vs. 21,05%) in fatigue group (p<0,05). Rare, mutated AA genotype of TNFα-308 gene was present only in fatigue group, and was absent in non fatigue group,and we found week significant difference (p=0,05) in allele frequency between these two group of patients.Conclusion: Based on these results, we concluded that proinflammatory gene polymorphism can predict the occurrence of fatigue in sarcoidosis patients.