TY - JOUR T1 - Expression of the β2-adrenoreceptor and M3-cholinoreceptor genes in patients with different severity of asthma and BHR level JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P1951 AU - Polina Selivanova AU - Evgeny Kulikov AU - Natalya Kirillova AU - Maxim Freydin AU - Ludmila Ogorodova Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P1951.abstract N2 - Background. Persistent airway inflammation, as well as nervous innervation of bronchial smooth muscles plays the main role in the formation of BHR, one of the key characteristic of asthma.Aim and objectives. To evaluate the β2-adrenoceptor (ADRB2) and M3-cholinoreceptor (CHRM3) gene expression in bronchial mucosa of patients with different severity of asthma. We hypothesized that the differential expression of these genes may contribute to the different BHR level.Methods. Biopsy specimens of right middle lobar bronchus were obtained from 30 asthma patients (13 with severe, 9 with moderate, and 8 with mild). The inclusion criteria followed the GINA, 2009. All subjects underwent a routine medical history taking, physical examination, spirometry, BHR measurement. The mRNA levels for the ADRB2 and CHRM3 genes in bronchial mucosa were revealed using quantitative RT–PCR (iQ SYBR Green Supermix, BioRad, USA), mRNA levels were then recalculated as 2-ddCt to the GAPDH mRNA.Results. An increase of the ADRB2 and CHRM3 genes expression was demonstrated in patients with severe asthma (ADRB2 mean=0.54; 95%CI 0.50-0.59; CHRM3 0.57; 0.53-0.60) as compared to patients with mild (ADRB2 0.34; 0.32-0.36; CHRM3 0.48; 0.46-0.49) and moderate disease (ADRB2 0.27; 0.25-0.29; CHRM3 0.46; 0.43-0.49) (p<0.05). It was revealed by correlation analysis that the level of the PC20 is negatively correlated with the mRNA levels of the ADRB2 and CHRM3 (R=-0.67 and -0.52, respectively; p<0.005), thus a high level of gene expression is associated with a high BHR.Conclusions. The differential expression of the ADRB2 and CHRM3 genes is associated with asthma severity and BHR level. ER -