RT Journal Article
SR Electronic
T1 Allergyprotective effects of Staphylococcus scuiri in a house dust mite (HDM) model of allergic airway inflammation
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P232
VO 40
IS Suppl 56
A1 Stefanie Hagner
A1 Min Zhao
A1 Hani Harb
A1 Harald Renz
A1 Petra Pfefferle
A1 Holger Garn
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P232.abstract
AB Background: Recently, epidemiological studies revealed a specific association between Staphylococcus scuiri exposure and reduced asthma prevalences.Aim: To investigate the influence and role of S. scuiri, a Gram-positive bacterium, on allergen-induced sensitization and airway inflammation in a murine model with a mixed phenotype (with eosinophil and neutrophil inflammation) using a clinically relevant allergen (HDM model).Methods: Mice were treated intranasally with S. scuiri three times a week before and during repeated intranasal exposures to HDM extract. Lung function, recruitment of inflammatory cells as well as production of related cytokines and chemokines were measured.Results: The application of S. scuiri strongly inhibited the generation of HDM extract-induced airway inflammation. Analyses of bronchoalveolar lavages showed a reduction of both, eosinophil and neutrophil numbers, in comparison to control-animals. The reduction of eosinophils correlated with a reduction of IL-5, and of goblet cell hyperplasia. The mRNA expression of eotaxin and KC, the key chemokines in the induction of eosinophils and neutrophils into allergic tissue, was markedly reduced.Conclusions: Administration of S. scuiri to mice challenged with HDM extracts protects them from the development of airway inflammation and associated pulmonary pathology.