PT - JOURNAL ARTICLE AU - Lingyan Chen AU - Jianguo Hong AU - Xiaojian Zhou AU - Xia Li AU - Zhen Li TI - The effect of 1,25-(OH)<sub>2</sub>D<sub>3</sub> supplementation on the expression of vitamin D receptor (VDR) on the lung of baby rats with asthma DP - 2012 Sep 01 TA - European Respiratory Journal PG - P1105 VI - 40 IP - Suppl 56 4099 - http://erj.ersjournals.com/content/40/Suppl_56/P1105.short 4100 - http://erj.ersjournals.com/content/40/Suppl_56/P1105.full SO - Eur Respir J2012 Sep 01; 40 AB - Aim: To investigate the effect of different doses of 1,25-(OH)2D3 supplementation on the expression of vitamin D receptor in the lung of baby rats with asthma. Methods Thirty two Wistar rats were randomly divided into four group and were given different doses of 1,25-(OH)2D3 except for the control group receiving normal saline. Then we chose eight weaned baby rats to establish asthma model. Expression of VDR was measured by immunochemistry and RT-PCR. Results: Light microscope showed that inflammation was less serious in medium and low dose group, but more seriously in high group.Immunohistochemistry indicated that the expression of VDR decreased in low and medium dose group, but increased in the high group(p&lt;0.01).View this table:The VDR expression in the lung of asmatic rat of different groupsRT-PCR were consistent with immunohistochemistry.View this table:The VDR mRNA expression in the lung of asmatic rats of different groupsConclusions Appropriately 1,25-(OH)2D3 supplementation decrease VDR expression and alleviate airway inflammation in baby rats with asthma, while overdosage supplementation might play deterimental effect.