RT Journal Article
SR Electronic
T1 Aggravation of bleomycin-induced pulmonary fibrosis in senescence-accelerated mouse
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3671
VO 40
IS Suppl 56
A1 Baohui Lou
A1 Qiao Ye
A1 Huaping Dai
A1 Kewu Huang
A1 Chen Wang
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P3671.abstract
AB Objective: Idiopathic pulmonary fibrosis (IPF) is predominantly a lung fibrotic disease of older adults, and the process underlying aging might significantly influence the development of pulmonary fibrosis. Bleomycin-induced lung injury was investigated in murine models of accelerated senescence (SAMP8) and of normal aging (SAMR1). The levels of Th1/Th2 related cytokine were also measured.Methods: Bleomycin or PBS was injected into the tracheal lumen of 12-month-old SAMP8, 4 and 12-month-old SAMR1 mice. Seven, 14 and 28 days after the injection, the mice were killed and the lungs were harvested for pathological examination, hydroxyproline assay and protein detect. Lung TGF-β1 expression was determined by western blot, and the levels of IL-4 and IFN-γ were detected by ELISA.Results: The aggravated bleomycin induced lung injury was observed in 12-month-old SAMP8 compared with 4 and 12-month-old SAMR1. Twenty eight days after injection of bleomycin, Ashcroft score was significant higher in 12-month-old SAMP8 than in 4 and 12-month-old SAMR1 (P<0.05, respectively). Seven days, 14 days and 28 days after bleomycin injection, lung TGF-β1 expression was increased in 12-month-old SAMP8 and SAMR1 compared with 4-month-old SAMR1. Similarly, the level of IL-4 and the IL-4/IFN-γ ratio of the lungs tended to be higher in 12-month-old SAMP8 and SAMR1 than in 4-month-old SAMR1, but the differences were not statistically significant.Conclusions: Bleomycin-induced pulmonary fibrosis in SAM was aggravated by aging. The old SAM with bleomycin-induced pulmonary fibrosis might be inclined to Th2-biased immune responses.(This work was funded by National Natural Science Foundation of China Grants 81070046).