RT Journal Article
SR Electronic
T1 Alterations of serum inflammatory biomarkers in the healthy and lung cancer patients before and post chemotherapy
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P4207
VO 40
IS Suppl 56
A1 Dawei Yang
A1 Xun Wang
A1 Tao Zeng
A1 Jie Hu
A1 Yuanlin Song
A1 Luonan Chen
A1 Xiangdong Wang
A1 Chunxue Bai
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P4207.abstract
AB Introduction: Only 20% of lung cancer patients could be early diagnosed with surgical treatment. Due to the high cost, it is not practical to apply CT scan and pathological biopsy for lung cancer as regular screening tools even in high-risk population. A simple but sensitive and specific assay used for lung cancer diagnosis and prognosis is warranted.Methods: Serum samples from 55 subjects including healthy people and patients with NSCLC (30 with adenocarcinoma and 13 with squamous cell carcinoma) were collected to measure 40 inflammatory mediators by multiplexed cytokine immunoassays. All patients have completed follow up for up to two years. A series of systematical computational analysis was applied.Results: The set of 17 cytokines (such as IL-9, CCL16, CXCL10, etc.) prefers to identify adenocarcinoma samples from pool of the population, while the set of 2 cytokines (MSPa and IL-29) prefers to recognize squamous cell carcinoma samples. The decision trees based on these two kinds of biomarkers can both achieve about 80% accuracy in leave-one-out cross-validation. Cytokines like CXCL5, CXCL10 and CCL16 were also found to play important roles in cancer survival. The co-expressed protein interaction network (CEPIN) of cytokines related to adenocarcinoma show dynamic convergent behavior during chemotherapy.Conclusions: This pattern of inflammatory mediators might be useful for cancer diagnosis, prognosis and evaluation of chemotherapy effects. The results of clustering of five CEPINs supported the adopted chemotherapy is effective for NSCLC patients.This study was supported by National Basic Research Program of China (973 Program) No. 2012CB933300.