PT  - JOURNAL ARTICLE
AU  - Natalya Kirillova
AU  - Elena Kremer
AU  - Ludmila Ogorodova
AU  - Ivan Deev
AU  - Evgeniy Kulikov
AU  - Olga Fedorova
AU  - Polina Selivanova
TI  - Low level of FoxP3 in bronchial asthma (BA) is associated with frequent exacerbations
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P1948
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P1948.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P1948.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Background. Regulatory T cells (T-reg) expressing FOXP3 inhibit the development of T cells towards Th2. In this work we hypothesized that FoxP3 is responsible for the development of exacerbations in asthma.Aim and objectives. To investigate the levels of CD4+FOXP3+ in moderate BA with different frequency of exacerbations compared to healthy donors.Methods. We included 20 patients with moderate asthma, which were divided into 2 groups. The first group (n=16) had at least 1 exacerbation of asthma during the previous year, the disease duration was 7,5 (3,0:12,0) years, FEV1 - 81,2 (72,2:89,4)%. The second group (n=4) with moderate BA had 2 or more exacerbations of asthma in the previous year, the duration of the disease was 14,5 (9,0:23,0) years, FEV1 - 78,4 (74,4:89,8)%. The control group was included 17 healthy adults. Peripheral blood mononuclear cells were isolated in Ficoll density. The circulating percentage of CD4+FoxP3+ T-regs in peripheral blood was estimated by the flow cytometry analysis (FACSCalibur Becton Dickinson, USA) using appropriate monoclonal antibodies.Results. It was found that moderate asthma is characterized by significantly lower CD4+FoxP3+ T-reg 0,99 (0,72:3,77)% compared to healthy control - 8,16 (7,66:9,42)% (p<0,01). The level of CD4+FoxP3+ was higher 1,8 (0,8:4,7)% in patients who didn't have the exacerbation of BA, or had a single acute of asthma compared to individuals, who had more than 2 exacerbation of asthma in the previous year - 0,53 (0,4:0,9), p=0,039.Conclusions. FoxP3 is responsible for the development of exacerbations in asthma. Possible, the low level of transcription factor FoxP3 is closely related to 2 or more exacerbations of BA per year.