RT Journal Article
SR Electronic
T1 The roles of PCDH1 on epithelial barrier function in the airway
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P1761
VO 40
IS Suppl 56
A1 Yutaka Kozu
A1 Yasuhiro Gon
A1 Akiko Sekiyama
A1 Masahiro Terakado
A1 Ikuko Takeshita
A1 Shuichiro Maruoka
A1 Shu Hashimoto
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P1761.abstract
AB [Background]PCDH1 is recently identified as a susceptibility gene of bronchial hypersensitivity (BHR). Although PCDH1 seems to be involved in the differentiation of the airway, the roles of PCDH1 on epithelial barrier functions have not been determined.[Methods]A human airway epithelial cell line, 16HBE, cells were cultured on Transwell chamber for 5days. Barrier function was evaluated by Trans Electric Resistance and dextran permeability. Expression of inflammatory cytokine was measured by RT-PCR. Morphology of cell junctions was analyzed by immunostaining using anti-PCDH1, anti-ZO-1, anti-Ocludin and an anti-E-cadherin antibody.[Results]The paracellular barrier function of 16HBE cell monolayer increased over time in culture. Similarly, the expression of PCDH1 increased for the period. The knockdown of PCDH1 significantly inhibited the paracellular barrier function. In addition, it inhibited the dsRNA-induced inflammatory cytokines expressions. Immunocytostaining revealed that PCDH might coexist with E-cadhein at the cell-cell contact sites.[Conclusion]Our results indicated that a susceptibility gene of PCDH1 plays an important roles on the immunological and physiological barriers of airway epithelium.