@article {ZhaoP1785, author = {Feng Zhao and Haofeng Ouyang and Changgui Wu and Zhikui Li}, title = {Association of glucocorticoid receptor gene polymorphisms of R23K and R477H and steroid-resistant asthma in Chinese Han population}, volume = {40}, number = {Suppl 56}, elocation-id = {P1785}, year = {2012}, publisher = {European Respiratory Society}, abstract = {Some previous studies have shown that steroid resistance in asthma is associated with the mutation of glucocorticoid receptor (GR) gene. Other studies, however, have reached the opposite conclusion. The aim of this study was to detect the GR polymorphisms of R23K and R477H in steroid-resistant (SR) asthma patients and steroid-sensitive (SS) asthma patients in Chinese Han population, and to elucidate the association between GR polymorphisms and steroid resistance in asthma. Sixty-four SR patients and 68 SS patients were recruited for the detection of R23K and R477H variants, using polymerase chain reaction-sequence specific primers. Cortisol contents in serum were examined. The equilibrium dissociation constants (Kd) were calculated by dexamethasone radioligand-binding assay and Scatchard analysis to determine GR affinity to glucocorticoids. No statistically significant difference was found in the distribution of R23K polymorphism between SR patients and SS patients. In R477H, the wild genotype GG and AA frequencies were significantly lower in SR patients than in SS patients (P=0.043). The cortisol content in serum was found no significant difference between SR patients and SS patients, and among different genotypes. No significant differences in Kd were found between GG genotype and GA genotype of R23K variant, GG genotype and GA+GG genotype of R477H variant. In SR patients, the Kd values were significant higher than in SS patients (P\<0.001). These findings suggest that GR polymorphisms of R23K and R477H exist in Chinese Han population. R477H variant is associated with steroid-resistant asthma in this population.}, issn = {0903-1936}, URL = {https://erj.ersjournals.com/content/40/Suppl_56/P1785}, eprint = {https://erj.ersjournals.com/content/40/Suppl_56/P1785.full.pdf}, journal = {European Respiratory Journal} }