TY - JOUR T1 - Role of altered level of oxidant-antioxidant in disease prognosis of asthma JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P806 AU - Asrar Ahmad AU - Mohammad Shameem AU - Qayyum Husain Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P806.abstract N2 - Background: Asthma is a chronic airway disorder which is associated to airway obstruction. Inflammatory and immune cells generate more reactive oxygen species in asthma and lead to contribute in tissue injury.Aims and objectives: The aim of this study was to investigate the role of oxidant-antioxidant imbalance with disease severity in asthmatic patients.Methods: In this study 140 asthmatic patients and 70 healthy controls were documented. Malondialdehyde level, total protein carbonyls and sulfhydryls were measured as the indicator of oxidative stress in plasma. Antioxidants were evaluated by the activity of catalase, glutathione peroxidase, total blood glutathione and total antioxidant capacity.Results: The present work showed that the levels of malondialdehyde (4.51±0.12 nmol/mL) and protein carbonyls (1.30±0.02 nmol/mg) were found to be significantly higher in asthmatic patients while protein sulfhydryls (0.54±0.01 mmol/L) decreased as compared to controls (2.84±0.12 nmol/mL, p<0.001; 0.79±0.02 nmol/mg, p<0.001 and 0.60±0.02 mmol/L, p<0.01 respectively). We also observed increased total blood glutathione (0.98±0.02 mmol/L), decreased glutathione peroxidase (41.22±1.10 U/g Hb) and catalase activity (4650±81.36 U/g Hb) in erythrocytes compared with control (0.84±0.04 mmol/L, p<0.01; 48.37±2.47 U/g Hb, p<0.01 and 4946±89.94 U/g Hb, p<0.05 respectively). The level of total antioxidant status (714.70±23.75 µmol/L) in plasma were also decreased as compared to control (840.40±28.39, p<0.001).Conclusions: These results support the hypothesis that an imbalance in oxidant-antioxidant is associated to the oxidative stress which plays a significant role in severity of the disease. ER -