TY - JOUR T1 - HRVC is the commonest rhinovirus group detected in children admitted to a paediatric intensive care unit with respiratory illnesses JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P4138 AU - Desmond Cox AU - Khoo Siew-Kim AU - Zhang Guicheng AU - Lindsay Katie AU - Geoff Knight AU - Anthony Keil AU - Jack Goldblatt AU - Ingrid Laing AU - Joelene Bizzintino AU - Peter Le Souef Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P4138.abstract N2 - Introduction: Respiratory viruses account for a significant proportion of acute admissions to the paediatric intensive care unit (PICU). Acute respiratory illnesses (ARI) represent 10-15% of all admissions to PICU. Recent studies have shown that human rhinovirus (HRV) was the most frequent virus detected in severe cases of ARI admitted to a PICU. There has been no study to date examining the prevalence of HRV groups (including the newly discovered HRVC group) in children admitted to a PICU.Aim: The aim of this study was to determine the prevalence of different HRV groups in children with respiratory illnesses admitted to a tertiary PICU.Methods: Nasopharyngeal aspirates (NPA) and clinical information were obtained from children admitted with respiratory illnesses to PICU between March 2009 and July 2011. RNA was extracted from NPA, and reversed transcribed. From cDNA, a 2-step PCR of the HRV 5′NCR was used for HRV detection, and sequencing for typing.Results: NPA from 229 children admitted to PICU were analysed. HRV was the commonest virus, being present in 93 (40.6%) samples examined, followed by respiratory syncytial virus (RSV) which was identified in 50 (21.8%) samples. Of the 77 NPA available for HRV typing, HRVC was found to be the commonest HRV group detected, representing 42 (54.5%) of the samples identified. HRVA group was found in 32 (41.5%) and HRVB in 3 (4%) of the samples analysed.Conclusions: This study demonstrated that HRV is the commonest virus identified in children admitted to a tertiary PICU with a respiratory illness. In addition, HRVC was the commonest HRV group detected across all respiratory illnesses. ER -