RT Journal Article
SR Electronic
T1 A single dose of a specific serinoprotease inhibitor attenuated the protease-antiprotease imbalance in an experimental model of emphysema
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P827
VO 40
IS Suppl 56
A1 Clarice R. Olivo
A1 Juliana D. Lourenço
A1 Luana Neves
A1 Francine Almeida
A1 Carla M. Prado
A1 Iolanda C.L. Tibério
A1 Aparecida Tanaka
A1 Sergio Sasaki
A1 Milton A. Martins
A1 Fernanda D.T.Q.S. Lopes
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P827.abstract
AB We showed that a single dose of specific serinoprotease inhibitor in mice attenuated parenchymal destruction with an improvement in lung function in emphysema. Objectives: To study the possible effects of this inhibitor in pathological mechanisms that contribute to emphysema. Methods: Mice were submitted to either a nasal instillation of porcine pancreatic elastase (PPE) or saline (S) and 1h after, animals received a second nasal instillation of either a protease inhibitor (r-BmTIA, 35.54pmol) or vehicle (VE). After 21 days,we evaluated the number of macrophages (MAC) and cells expressing metalloproteinase 12 (MMP12) to assess the protease-antiprotease imbalance. We quantified caspase-3 to evaluate apoptosis and expression of 8-isoprostane as a marker of oxidative stress. Results: We observed an increase in MAC in groups that received PPE and only PPE-VE group showed higher values of MMP-12. There were no differences between groups when we analyzed caspase-3 and 8-isoprostane.View this table:Conclusions: Although this inhibitor treatment has not diminished the macrophages in animals that received PPE, it reduced MMP-12 positive cells, suggesting that the improvement in parenchyma destruction and in lung function occured due to attenuation in protease-antiprotease imbalance.Supported by FAPESP, LIMHC-FMUSP, Brazil.