RT Journal Article
SR Electronic
T1 Late stage of experimental pulmonary fibrosis is modulated by collagen V
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 2826
VO 40
IS Suppl 56
A1 Alexandre Todorovic Fabro
A1 Mozar Suzigan de Almeida
A1 Igor O. Minatel
A1 Erika C. Prado
A1 Claudia Aparecida Rainho
A1 P.R. Teodoro Walcy
A1 Edwin R. Parra Cuentas
A1 Vera Capelozzi
A1 Helmut Popper
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/2826.abstract
AB Background: The IPF is a disease with high morbi-mortality. Several experimental models of pulmonary fibrosis (PF) has been proposed, however, a later stage of these models tend to go for the resolution of the fibrosis, but in different degrees of intensity depending on the strain. Thus these mechanisms in certain strains may participate in the progression of PF.Aims: To study the immune-fibrotic pattern in different models of PF in the late stage (21d).Methods: We used the models of Bleomycin-Balb/c (BLM), Paraquat-Balb/c, Bleomycin-C57BL/6 and BLM-IL17RA-KO-C57BL/6. We analyzed the amounts of total collagen (TC) and collagen V (Col5) through the morphometric evaluation by the picrosirius and IF. These data were validated by RT-PCR of Col5.Results: The peribronchiolar TC by PPM did not differ between the treated groups, but the peripheral interstitial TC was higher in the C57BL/6, independent of the absence of IL-17RA. The protein expression of Col5 was higher in IL-17RA-KO (75,5±9% X 52,7±13%; p=0,01) and lower in BLM-Balb/c (69,8±3,4% X 53,3±14,3%; p<0,05). Likewise, the gene expression of Col5 was also higher in the IL17RA-KO (p<0.0485) and lower in the BLM-Balb/c (p<0.0037) (Figure 1).Conclusion: The perpetuation of PF in fibrosis-susceptible mice is related to expression of Col5 in a IL-17-independent manner and this suggests that Col5 is an important component responsible for the development of PF.