TY - JOUR T1 - Myocardial prostacyclin effects in experimental acute afterload-induced right ventricular failure JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P3164 AU - Celine Dewachter AU - Laurence Dewachter AU - Marie Vercruyssen AU - Benoit Rondelet AU - Dean Paul Schraufnagel AU - Serge Brimioulle AU - Francois Kerbaul AU - Robert Naeije Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P3164.abstract N2 - It has been suggested that prostacyclin improves patients with pulmonary hypertension through direct myocardial effects. In an experimental model of acute right ventricular (RV) failure on increased afterload, we previously reported that prostacyclin did not present with significant inotropic effects, but that there might have been a trend as assessed from improved RV-arterial coupling (Kerbaul et al. AJRCCM 2007).Therefore, we further explored the pathobiology of acute RV failure with/without prostacyclin infusion.Dogs were randomized to a sham-(n=8) or to a 90-min pulmonary artery constriction (PAC)-operation (n=15). In 7 dogs with persistent RV failure, prostacyclin was infused 30-min after banding release. After euthanasia, RV tissue was sampled for pathobiological evaluation.90-min PAC increased RV gene expressions of interleukin (IL)1β, monocyte chemoattractant protein (MCP)1, IL6, VCAM1, and decreased expression of IL10. No changes in RV gene expressions of IL1α, macrophage inflammatory protein (MIP)1α and ICAM1 were observed after PAC. Protein expressions of IL1β and IL6 were increased in the failing RV, while IL10 protein expression was decreased. Pro-inflammatory IL6/IL10 and pro-apoptotic Bax/Bcl2 ratios were increased in the failing RV. Increased diffuse macrophage recruitment was observed within the failing RV. Prostacyclin decreased RV gene expressions of IL1β, MCP1, MIP1α, VCAM1 and increased IL10 expression. Prostacyclin decreased RV gene IL6/IL10 and Bax/Bcl2 ratios, and IL1β protein expression compared to PAC group.Acute load-induced persistent RV failure appears to be related to an activation of inflammatory processes which seems to be limited by prostacyclin. ER -