TY - JOUR T1 - Use of immune cell function assay in immune surveillance of lung transplant recipients: Correlations with clinical outcome and other immune assays JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P2441 AU - Francesco Bini AU - Annamaria Grasso AU - Simona Miserere AU - Rita Di Domenica AU - Monica Morosini AU - Tiberio Oggionni AU - Manuela Cova AU - Luisetti Maurizio AU - Federica Meloni Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P2441.abstract N2 - Introduction: Infection still remains a significant cause of morbidity after lung transplantation. The Cylex ImmuKnow assay (IKA), provides a global assessment of CD4 T cell immune function to help monitor the immune reactive capacity of graft recipients. We use IKA in the immunological follow-up of lung recipients. We assess clinical utility of IKA and correlate its results with other immunological parameters.Methods: IKA was always performed on clinical demand. From October 2008 to February 2012 we performed IKA on 110 samples. The values of CD4+ T cell activation were classified as strong (> than 525ng/mL of ATP), moderate (525<>225) and low (< 225). Than we correlated a subgroup of 74 IKA samples with CD4+ cells, CD8+ cells, ratio CD4+/CD8+, NK cells, CD4+CD25highCD127-/dimcells in blood and BAL.Results: High correlation between lower values and the occurrence of infectious episodes was observed (p<0.05).View this table:Number of infections and IKA levelsInstead, no correlation was found with the biopsy confirmed diagnosis of acute rejection.Values of IKA did not correlate with levels of tacrolimus (P=0,8).Similarly, only a trend toward an inverse relationship was present with CD4+ cell counts.As for Treg cells counts, again no significant correlation was present.Of note in 81% of low IKA results, the test was the basis of a change in the immunosuppressive regimen.Conclusion: IKA values are helpful in identifying patients at high infectious risk and can be used to adapt IS regimen to patient need. ER -