PT - JOURNAL ARTICLE AU - Yijie Zhang AU - Yusheng Chen AU - Yuqian Li AU - Zhanwei Wang AU - Machao Li AU - Daxuan Wang AU - Ying Shang AU - Rongrong Fu AU - Zhancheng Gao TI - Serotyping, multilocus sequence typing and antibiotic resistance of <em>Streptococcus pneumoniae</em> isolates in China DP - 2012 Sep 01 TA - European Respiratory Journal PG - P562 VI - 40 IP - Suppl 56 4099 - http://erj.ersjournals.com/content/40/Suppl_56/P562.short 4100 - http://erj.ersjournals.com/content/40/Suppl_56/P562.full SO - Eur Respir J2012 Sep 01; 40 AB - Introduction Streptococcus pneumoniae is a major causative agent of severe infections, which has become a major public health concern.Objectives A prospective study was performed to investigate the serotype distribution, genetic relationship and antimicrobial resistance in Streptococcus pneumoniae (S. pneumoniae) isolates from patients in China.Methods 21 Blood/cerebrospinal fluid (CSF) isolates and 18 sputum isolates were collected from patients admitted to the Peking University People's Hospital and Fujian Provincial Hospital were analyzed for investigating the serotypes, the multilocus sequence typing (MLST), and their susceptibilities to antibiotics.Results The most frequent serotypes of blood/CSF isolates were 14 (38.1%), 19A (14.3%), 23F (9.5%), 18C (9.5%), and in the sputum isolates the most frequent serotypes were 19F(33.3%), 23F(16.7%), 19A(11.1%), 3(11.1%). The coverage rates of the blood/CSF and sputum isolates for the 7-valent pneumococcal conjugate vaccine (PCV7) were 66.7% and 61.1%, respectively. 4 of 5 isolates determined as 19A were collected from patients &lt;5 years old. The MLST analysis showed that all the 5 isolates were ST320. All the 21 blood/CSF isolates, the resistance rates of erythromycin and penicillin were 90.5% and 61.9%. Of the 18 sputum isolates, the resistance rates of erythromycin and penicillin were 83.3% and 66.7%. All of the isolates of serotype 19A were resistant to both penicillin and erythromycin.Conclusions The introduction of PCV13, in which the 19A conjugate is included, will be necessary in China because of the high rates and severe antibiotic resistance of 19A.