TY - JOUR T1 - Evaluation of cell spectrum in induced sputum of young cigarette smokers JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P4064 AU - Agnese Kislina AU - Gunta Strazda AU - Liga Balode AU - Zane Sinkevica AU - Darja Isajeva AU - Sergejs Isajevs AU - Normunds Jurka AU - Immanuels Taivans AU - Valentina Gordjusina AU - Viesturs Silins Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P4064.abstract N2 - It is of great importance to be able to diagnose early inflammatory changes that can lead to COPD in airways of young cigarette smokers.Aim: The aim of this study was to analyze the cell spectrum of induced sputum in young cigarette smokers, with emphasis on T-regulatory cells.Method: 20 healthy non-allergic smokers (Mean Age - 22±0.84; regular usage - 2.60±1.18 pack-years), and 20 non-smokers gave informed consent to participate in the study. Lung function measurements, sputum induction (IS) and sputum cell analyses were preformed.Results: Demographic data for both study groups did not differ significantly. Both non-smokers and smokers had normal lung function indices. Induced sputum of smokers contained higher (p = 0.021) absolute amount of neutrophils [29.70 (20.96 – 42.09), cellsx104/mL], compared with non-smokers [16.51 (11.65 – 23.39) cellsx104/ mL]. FOXP3-positive T reg cells in sputum of young smokers showed a trend towards statistically significant increase [11.10 (7.75 – 15.88) cellsx104/ mL], compared with non-smokers [6.79 (4.75 – 9.72) cellsx104/ mL, p = 0.057]. Regression analysis showed significant dependency of FOXP3-positive T reg cells (r2 = 0.103; p = 0.044), neutrophils (r2 = 0.179; p = 0.007) and macrophages of induced sputum (r2 = 0.118; p = 0.030) from the number of smoked pack–years.Conclusion: This study demonstrates that young smokers have early inflammatory changes in their airways, which not only initiate non-specific mechanisms recruiting neutrophils, but also involve specific immune mechanisms with recruitment of T reg lymphocytes. The lymphocyte response is probably adaptive. ER -