PT  - JOURNAL ARTICLE
AU  - Dora Marinova
AU  - Yanina Slavova
AU  - Plamen Titorenkov
AU  - Vladimir Maksimov
AU  - Nedka Trifonova
AU  - Vladimir Djambazov
AU  - Maja Tafradjiiska
AU  - Danail Petrov
TI  - Immunohistochemical expression of Hsp27 and alphaB-crystallin in different histological subtypes of lung cancer
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P1213
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P1213.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P1213.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Aim: to evaluate the immunohistochemical expression of heat shock protein 27 (Hsp27) and alphaB-crystallin (Cryab) in resected lung cancers.Methods: Surgically resected specimen from 105 patients (men n=72; women n=33, age 57± 12) with lung cancer were studied: carcinoid tumors(CT)-35, small cell lung carcinoma(SCLC)-20, large cell neuroendocrine carcinoma(LCNEC)-10, adenocarcinoma(AC)-20, and squamous cell carcinoma(SCC)-20. The histological subtype, pTNM stage, and the immunohistochemical expression of Hsp27 and Cryab were evaluated.Results: The distribution in stages was as follows: CT-I stage-25(71%),II-7(20%),IIIA-3(9%); SCLC-I-7(35%), II-4(20%), IIIA-8(40%), IV-1(5%); LCNEC-I-4(40%),II-5(50%),III-10(10%); AC-I-8(40%),II-2(10%),III-10(50%); SCC-I-8(40%),II-1(5%),III-11(55%) (p<0.001). Hsp27 cytoplasmic expression was observed in 97% of tumors (71% of all cases express Hsp27 in >75% ot tumor cells),and in combination with nuclear expression in 46%. Nuclear expression was in: 100% of LCNEC,50%-SCLC and SCC,35%-AC, 29%-CT (p<0.001). Significant correlation between citoplasmic expression of Hsp27 and pTNM stage was observed (p<0.05).Nuclear expression of Cryab was observed in 99%,and in combination with cytoplasmic-in 23% (65%-AC,17%-CT,10%-SCC,10%-LCNEC,5%-SCLC;p=0.001). Significant correlations between nuclear expression of Cryab and N-status(p<0.001),pTNM stage(p=0.02),tumor size(p=0.04); and between citoplasmic expression of alphaB-crystallin and pTNM stage (p<0.001) were observed.Conclusion: low weight heat shock proteins play a major role in cancerogenesis of lung cancer, eventually has a prognostic value and may be used as targets for therapy.