TY - JOUR T1 - Th1/Th2/Th17-related cytokines in the bronchoalveolar lavage fluid of patients with sarcoidosis in relation to smoking JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P3611 AU - Tomasz Urbankowski AU - Grazyna Hoser AU - Rafal Krenke AU - Joanna Domagala-Kulawik Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P3611.abstract N2 - Sarcoidosis (SA) a multiorgan granulomatous disease. The inflammatory process in involved organs is characterized by Th1/Th2 imbalance and participation of Th17 cells, what was recently documented. SA is more prevalent among non-smokers than in smokers, however the impact of cigarette smoking on inflammation in SA remains unclear.The aim of the study was to evaluate the Th1/Th2/Th17-related cytokine concentrations in bronchoalveolar lavage fluid (BALf) of patients with pulmonary SA in relation to smoking status. 61 SA patients were investigated (26 ever (S) and 35 never smokers (NS)). The concentrations of IL-17A, INFγ, TNFα, IL-10, IL-6,IL-4 and IL-2 were measured by flow cytometry using Cytometric Bead Array method.The concentration of TNFα, IL-10, IL-4 and IL-2 was below minimum of detection in most cases. There was significant correlation of INFγ concentration with number of macrophages and between IL-6 concentration with number of neutrophils (R=0.6, P<0.05, r=0.3, P<0.05, respectively).We found higher concentration of INFγ and lower of IL-6 in S than in NS (1.28 vs 1.23 and 1.66 vs 2.38, respectively, difference nonsignificant). The mean concentration of IL-17A was 6.1 pg/mL and did not differ between S and NS. There was significant correlation of IL-17A with IL-6 concentration (R=0.9, p<0.05) in the whole group and in S group. The concentration of IL-6 correlated negatively with the results of pulmonary function tests.Our observation shows a slightly different profile of cytokine concentration in the BAL of smoking patients with SA. The lack of significance may be related to the short smoking history of patients. ER -