TY - JOUR T1 - Association analysis of Β2-adrenergic receptor gene polymorphisms (Arg16Gly and Gln27Glu) with asthma in the Volga-Ural region of Russia JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P474 AU - Yuliya Fedorova AU - Alexandra Karunas AU - Galiya Gimalova AU - Nailya Ramazanova AU - Regina Murzina AU - Lilya Muchtarova AU - Elena Galimova AU - Radik Gatiyatullin AU - Shamil Zagidullin AU - Esfir Etkina AU - Elza Khusnutdinova Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P474.abstract N2 - The β2-adrenergic agonists are the most potent bronchodilators for the treatment of asthma. Genetic variation in the ADRB2 gene has been hypothesized to have a role in differential response to beta-agonist (BA) therapy in asthma. Two polymorphic variants rs1042713 (Gly16Arg) and rs1042714 (Gln27Glu) were genotyped in 618 patients with physician-diagnosed asthma, aged 2-60 years (192 Russians, 139 Tatars, 82 Bashkirs and 205 mixed origins), and 366 nonasthmatic individuals (123 Russians, 91 Tatars, 51 Bashkirs and 101 mixed origins) from the Volga-Ural region of Russia. Genotypes were determined by the PCR-RFLP method. Data were analyzed using the chi-square test with Haploview software. We found significant association of Gln27Glu polymorphism with mild decrease FEV1 (60-80% of the predicted value) in Russian patients. The frequencies of genotype ADRB2*27Gln/27Gln and allele ADRB2*27Gln were increased in asthmatics of this group compared to controls (p=0,03, OR=2,25 (95%CI 1,05-4,82) and p=0,02, OR=1,97 (95%CI 1,11-3,50), accordingly). The analysis of Arg16Gly polymorphism showed significant association with moderate asthma in Tatars. The higher frequency of the ADRB2*16Gly/16Arg heterozygotes was revealed in the patients compared with controls (p=0,02, OR=2,25 (95%CI 1,15-4,03)). In summary, these results suggest an important role for polymorphisms of gene ADRB2 in the development of asthma in the Volga-Ural region of Russia. ER -