TY - JOUR T1 - Systemic oxygenation affects post-transplantation edema formation and pulmonary artery hypertension in an ex vivo animal model JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P1482 AU - Sara Klein AU - Stefan Dhein AU - Luisa Bauer AU - Franziska Schlegel AU - Sven Lehmann AU - Markus Barten AU - Friedrich-Wilhelm Mohr AU - Hartmuth Bittner Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P1482.abstract N2 - Introduction: By using an ex vivo model of isolated perfused and ventilated rabbit lungs we investigated the influence of systemic oxygenation on pulmonary function during simulated transplantation.Methods: Lungs of New Zealand White rabbits were flush-perfused with Perfadex® Solution, followed by an ischemic storage for 4h on ice. Thereafter ventilation and reperfusion for 2h were continued to simulate a transplantation situation (oxygenated group, pulmonary artery pO2=120mmHg). In another series the perfusate inflow was gassed with nitrogen to simulate the typical situation with deoxygenated pulmonary artery blood and not reanastomized private vessels(deoxygenated group, pulmonary artery pO2=50mmHg). Hemodynamic and ventilatory parameters were continuously detected.Results: After 2h reperfusion time the oxygenated group showed a significant lower PAP and lung weight compared to the deoxygenated group (p<0.05). PAP and lung weight steadily increased after reestablishment of lung perfusion (PAP 8.78±0.89 to 11.5±1.06cmH2O; lung weight 22.1±1.32 to 35.4±4.23g). This development was significantly influenced by the intravascular pO2 (PAP 9.65±0.43 to 8.02±0.63cmH2O; lung weight 17.9±1.54 to 21.5±2.29g, p<0.05).Conclusions: Oxygenation of the lung perfusate during simulated transplantation attenuates post transplant edema formation and decreases pulmonal arterial hypertension. Transfering this to the surgical situation, revascularisation of bronchial arteries after lung transplantation might initiate positive effects in the early phase after lung transplantation. ER -