TY - JOUR T1 - T regulatory cells in exacerbation control of chronic obstructive pulmonary disease of mild and moderate stage JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P2310 AU - Oleg Enikeev AU - Svetlana Enikeeva AU - Damir Enikeev Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P2310.abstract N2 - Aim: to investigate the levels of peripheral CD4(+)CD25(++) regulatory T cells and the inflammatory and anti inflammatory cytokines in exacerbation dynamics of mild and moderate stage of chronic obstructive pulmonary disease (AECOPD) patients.Methods: Peripheral blood samples were collected from 20 patients with mild AECOPD and 21 patients with moderate AECOPD, aged (55,6±3,2; 51±3 respectively) and 20 man from control group. Lymphocytes were isolated by two-color labeled monoclonal antibodies flow cytometry to examine the quantities and percentage of CD4+CD25+ T cell, CD4+CD25++ (CD4(+)Treg), CD3+CD19- T cell, CD3+CD4+ T cell, CD3+CD8+ T cell, respectively. Enzyme immunodetection was used to detect the expression of interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-alpha), procalcitonin (PCT) and C-reactive protein (CRP). Mann-Whitney test was used for comparison between data before and after treatment and control group.Results. In patients with mild and moderate AECOPD take place significant decreasing of Tregulatory cells (CD4+CD25++) from 3,3±0,8% in control to 1,13±0,17% and 1,3±0,12%%, respectively (p<0,01). Interestingly that percentage of Treg didn't change in dynamics of conventional therapy. Reliable increase of TNF-α and decrease of PCT take place in both groups (p<0,01). Neveretheless CRP and Il-10 levels of patients with COPD don't differ from the same values of control group. Conclusion. The decrease of Treg response and the lost balance between inflammatory/antiinflammatory cytokines, suggest a lack of regulation of the systemic inflammatory response that may contribute to pathogenesis in AECOPD of mild and moderate stage. ER -