PT  - JOURNAL ARTICLE
AU  - Valeriy Mineev
AU  - Lada Sorokina
AU  - Michael Nyoma
AU  - Georgiy Lipkin
AU  - Valeria Lim
AU  - Anna Eremeeva
AU  - Yulia Malfygina
AU  - Vasiliy Ivanov
AU  - Vasiliy Trofimov
TI  - The role of transcription factor PAX-5 (BSAP) in asthma severity and in the activity of allergic inflammation
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - P779
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/P779.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/P779.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - PAX-5 is transcription factor of B-cell, which is crucial in asthma pathogenesis.The aim is to evaluate PAX-5 role in allergic (AA) and non-allergic asthma (NAA).Materials and methods. Peripheral blood lymphocytes from 107 asthma patients and 22 healthy were examined. Part of lymphocytes was analyzed after 24h incubation with and without IL-4 10 ng/ml. To estimate PAX-5 and CHε (constant parts of IgE heavy chains) mRNA semi-quantitive RT-PCR was performed.Results: PAX-5 mRNA levels were significantly increased in patients with NAA in comparison with healthy (p=0,018) and AA patients (p=0,03) (U-crit.). We revealed important positive correlations of PAX-5 with CHε which were stronger in patients with normal and low serum IgE (≤150 MU/ml) than in patients with high serum IgE levels (>150 MU/ml) (r=0,543; p<0,001; n=53 and r=0,474; p=0,017; n=25 resp.). We revealed significant negative correlations of PAX-5 with sputum leucocytes and eosinophiles amount in patients with AA (ρ=-0,390; p=0,040; n=28 and ρ=-0,385; p=0,043; n=28 resp.).Significant decrease of mRNA PAX-5 was registered in lymphocytes from AA patients after 24h IL-4 action (p=0,046; n=13, W-crit.). This decrease was not significant in healthy (p=0,345; n=5, W-crit.).Conclusion: We revealed the association of PAX-5 and NAA development. It may be explained by fact of PAX-5 prolonging B-cell life and its functioning as antigen-presenting cell. In patients with AA PAX-5 may be considered as protective factor. IL-4 may influence PAX-5 expression through the pathways which are involved in asthma pathogenesis (e.g. STAT6 signaling).The work was supported by Saint-Petersburg government grant (No. 4/04-05/1-A).