RT Journal Article
SR Electronic
T1 Comparative anti-oxidant effects of carbocysteine and fluticasone propionate in cigarette smoke stimulated airway epithelial cells
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP P3717
VO 40
IS Suppl 56
A1 Elisabetta Pace
A1 Maria Ferraro
A1 Serena Di Vincenzo
A1 Rossella Balsamo
A1 Luigi Lanata
A1 Andreina Bruno
A1 Luana Lipari
A1 Mark Gjomarkaj
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/P3717.abstract
AB Cigarette smoke extracts (CSE) induce oxidative stress, an important feature in chronic obstructive pulmonary disease (COPD). Oxidative stress contributes to the poor clinical efficacy of corticosteroids. Carbocysteine, an anti-oxidant and mucolytic agent, is effective in reducing the severity and the rate of exacerbations in COPD patients. The effects of carbocysteine on CSE induced oxidative stress in nasal and in bronchial epithelial cells as well as the comparison of these anti-oxidant effects of carbocysteine with those of fluticasone propionate are largely unknown.The present study was aimed to assess:the effects of carbocysteine (10-4 M) in cell survival and intracellular reactive oxygen species (ROS) production in CSE stimulated bronchial (16-HBE) and in CSE stimulated nasal (RPMI 2650) epithelial cells and to compare these effects with those of fluticasone propionate (10-8 M).Carbocysteine or fluticasone propionate did not induce cell necrosis (propidium positive cells) or cell apoptosis (Annexin V positive/propidium negative cells) in 16 HBE and in RPMI 2650 at the tested concentrations. CSE increased intracellular ROS production in 16HBE and in RPMI 2650. Fluticasone propionate was not able to significantly reduce intracellular ROS production in both 16HBE and in RPMI 2650. Carbocysteine was able to significantly reduce intracellular ROS production and was more effective than fluticasone propionate in reducing these CSE mediated effects in both cell lines.In conclusion, the present study provides compelling evidences that carbocysteine may be considered a promising strategy in diseases associated to corticosteroid resistance.