PT  - JOURNAL ARTICLE
AU  - Martha Bustos
AU  - Maria Kapetanaki
AU  - Ernest Meyer
AU  - Albert Donnenberg
AU  - Lyle Mroz
AU  - Naftali Kaminski
AU  - Ana Mora
AU  - Mauricio Rojas
TI  - LSC 2012 abstract - Age-related changes in the relative expression of functional genes in mesenchymal stem cells
DP  - 2012 Sep 01
TA  - European Respiratory Journal
PG  - 4528
VI  - 40
IP  - Suppl 56
4099  - http://erj.ersjournals.com/content/40/Suppl_56/4528.short
4100  - http://erj.ersjournals.com/content/40/Suppl_56/4528.full
SO  - Eur Respir J2012 Sep 01; 40
AB  - Age-associated changes increased susceptibility to a variety of lung pathologies. Recently, mesenchymal stem cells or B-MSCs have emerged as a critical reparative response mechanism to lung injury. We had demonstrated an association, in animal models, between age and an increase in the susceptibility to injury and we had identified functional differences between B-MSCs from young and old mice.In the present study, we examined the consequences of aging in the gene expression.METHODS: 3 and 24 months old B6 mice were sacrificed and B-MSC were isolated according to the expression of the surface markers. RNA was obtained after sorting and used for hybridization on Agilent gene expression microarrays. Ingenuity software was used to determine functional enrichment. For statistical analysis we applied significance analysis of microarrays. A qvalue of 5, which corresponds to a 5% false discovery rate, was used as a cutoff of statistical significance. Gene validations were performed by RT-PCR.RESULTS: We demonstrate that aging induce a decrease in the gene expression on B-MSC. The mechanisms affected by the decrease on gene expression includecellular trafficking, cellular growth and proliferation.CONCLUSION: Old B-MSCs have a different expression profile that exhibits a decrease in the expression of genes that control importan B-MSC functions.