RT Journal Article
SR Electronic
T1 LSC 2012 abstract - Neutrophil trafficking in acute lung injury: A novel human ex vivo model
JF European Respiratory Journal
JO Eur Respir J
FD European Respiratory Society
SP 4318
VO 40
IS Suppl 56
A1 Alastair Proudfoot
A1 Charlotte Summers
A1 Timothy Jackson
A1 Matthew Hind
A1 Mark Griffiths
YR 2012
UL http://erj.ersjournals.com/content/40/Suppl_56/4318.abstract
AB Activation and migration of neutrophils into the lung is a central factor in both the onset and progression of acute lung injury (ALI). The assessment of neutrophil biology and trafficking in the lung is fraught with methodological pitfalls.Human ex vivo ALI models could provide a tractable platform with which to investigate neutrophil trafficking in the lung.Human lungs from brain dead donors (N=3) were cold preserved and transferred to our institution. Lungs were placed on an ex vivo lung perfusion circuit and following rewarming, E Coli lipopolysaccharide (LPS, 6mg) was injected intra-bronchially into the RML. The LLL was used as the control. CT images, tissue biopsies and BALF for cytopsin preparations were taken at T=0 and 4 hours after LPS injury.CT Images showed ground glass infiltrate in the RML at 4 hours with otherwise normal lung by CT criteria (Figure 1). Compared to control lung (Figure 2A), at 4 hours, LPS injured lung (Figure 2B) demonstrated a neutrophilc alveolitis while BAL cytopsin of LPS injured lung showed a predominance of neutrophils (Figure 2C).Further characterisation of this novel model allied with established methodology in pulmonary neutrophil trafficking will provide a powerful tool to investigate neutrophil biology in human ALI.Fig.1 CT image of LPS injured lung at 4 hoursFigure 2