TY - JOUR T1 - Change of phenotype and phenotypic plasticity of alveolar macrophages in COPD JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P811 AU - Svetlana Lyamina AU - Sergey Kalish AU - Igor Malyshev Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P811.abstract N2 - Development of chronic obstructive pulmonary disease (COPD) is due to imbalance of Th1/Th2 immune responses which suppose disorder in M1 and M2 macrophages phenotypes. Objective:assessment of macrophages phenotype and phenotypic plasticity of alveolar macrophages (AM) in COPD. Methods: In vitro experiments were carried out on AM cultures of COPD patients (n=18, 59.7±3.56 y.o.). AM phenotype was assessed by flow cytometry (Beckman Coulter, FC500) by surface macrophages markers CD80, CD25 and CD163, CD206, typical for M1 and M2 phenotype, respectively. Phenotypic plasticity of AM was measured as percentage change of markers during 36 hours of AM reprogramming in the presence of standard serum (FBS) in concentrations 0%, 10%, 40%. Results.In COPD patients AM of M1 phenotype prevailed, thus AM population was not monophenotypical: CD80 44.76%±1.80%, CD25 70.26%±2.20%, CD163 31.71%±1.74% and CD206 37.5%±1.23%. During AM reprogramming decreasing of FBS concentrations from 40% to 0% significantly changed expression of M1 phenotype markers: CD80 from 38.09%+0.93% to 65.42%+1.07% and CD25 from 41.51%+1.24% to 72.36%+1.43%. Increase of FBS concentrations from 0% to 40% significantly increased M2 markers expression CD163 and CD206. After 36 hours of culturing with 10%FBS only CD80 pointed to M1 phenotype prevalence in AM of COPD patients. Conclusions: In changing environment the previous condition of macrophages phenotype is longer fixed by M1 marker CD80, and CD80 can be used for phenotypic plasticity definition. Phenotypic plasticity should be assessed specific to certain factors so that different markers show different picture of phenotypic plasticity in COPD. ER -