TY - JOUR T1 - Cigarette smoke induces a distinct fibrotic signature in bronchial epithelial cells JF - European Respiratory Journal JO - Eur Respir J VL - 40 IS - Suppl 56 SP - P4584 AU - Brian Oliver AU - David Krimmer AU - Ling Chen AU - Janette Burgess AU - Judith Black AU - Qi Ge Y1 - 2012/09/01 UR - http://erj.ersjournals.com/content/40/Suppl_56/P4584.abstract N2 - Rationale: We have previously shown cigarette smoke extract (CSE) induces the production of the extracellular matrix proteins fibronectin and perlecan in human lung fibroblasts (Krimmer et al AJRCMB 2011). The proteins were also induced by transforming growth factor β (TGFβ); however the signal transduction pathways activated by CSE were different to those used by TGFβ. These differences lead us to speculate that if we were to look at an increased number of molecules involved in the development of fibrosis, we would find differential upregulation by TGFβ and CSE.Aim To compare fibrosis related genes upregulated by TGFβ or CSE in primary human bronchial epithelial (HBE) cells.Methods: HBE cells were grown to confluence in BEGM in the absence or presence of 5ng/ml of TGFβ1 or 5% CSE. Total RNA were collected after 72 hours. The expression of fibrosis related genes was measured by real time PCR. In total we examined 85 fibrosis related genes. A cut off of a minimum of 1.5 fold induction was used to indicate upregulation of a gene.Results: TGFβ upregulated 49 different fibrosis associated genes, CSE upregulated only 16, and of all the upregulated genes, 4 were uniquely upregulated by CSE (catenin β1, extracellular matrix protein 1, and TIMP 1 and 2). As expected, TGFβ upregulated a variety of ECM proteins (eg collagen I, IV-VIII, fibronectin); integrins (eg integrin α2, α4-6), and degradative enzymes (eg matrix metalloproteinase (MMP) 1-3, 9-16). In contrast, CSE upregulated only two collagens (XI and XIV) and MMP9.Conclusion: As CSE selectively upregulated a subset of TGF-β induced genes, elucidation of the specific mechanisms involved is likely to give novel insight into the pathophysiology of COPD. ER -