RT Journal Article SR Electronic T1 The role of pequi fruit (Caryocar brasiliense Camb) pulp oil, as a natural source of antioxidants, in experimental lung cancer JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP P4194 VO 40 IS Suppl 56 A1 Natália Beatriz Rigoldi Colombo A1 Edwin Roger Parra A1 César Koppe Grisólia A1 Márcia Hage A1 Denise Frediani Barbeiro A1 Vera Luíza Capelozzi YR 2012 UL http://erj.ersjournals.com/content/40/Suppl_56/P4194.abstract AB Background: Caryocar brasiliense Camb, most known as pequi, is a Brazilian fruit that has high levels of antioxidants properties. The aim of this study was to evaluate the antioxidant activity of the pequi oil. Methods: 18 male BALB/c mice was studied: 14 animals received by gavage 0,5μL/mg/day of pequi oil (Control+CBCoil = 4) during 75 days. After 15 days, 10 mices received two doses of 1,5g/kg intraperitoneal of urethane (Urethane+CBC oil=10). The other 4 animals were only submitted to the two doses of urethane (Urethane group=4). After 75 days, these groups were sacrificed. Antioxidant activity of pequi oil was evaluated in the lung tissues by the biochemical TBARS test(Thiobarbituric acid-reactive substances) and DNA damage by the comet test method. Results: The lung parenchyma from the Urethane groups without oil and with oil showed neoplasic formations induced by the chemical carcinogenesis in contrast with Control + CBC oil group.The image analysis of the comet assay showed a statistical significant decreased of the DNA damage cells in the Urethane + CBC oil group when compared with urethane group. TBARS test showed a significant decreased of the lipid peroxidation in the Urethane + CBC oil, similar as values of the Control+CBC oil, when compared with Urethane group. Conclusion: We conclude that the different natural antioxidant components found in the pequi oil are efficient to diminish the oxidative stress status and the DNA damage in chemical carcinogenesis induced by urethane experimental lung cancer, suggesting that this type of strategies may have a greater impact in lung cancer treatment. Financial Support: FAPESP, CNPq.